Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblasts)
Specification
fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - Methanol, 30 min at -20°C
Blocking step
FCS as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: RT°C
Other product details
Concentration
4 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 anti-mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ioannis Gavvovidis
Verified customer
提交于 Dec 02 2010