Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
Yes - Triton X-100
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Dianova 711-166-152
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Blue: Dapi (nuclei), Green: FITC-Avidin (mastcells), Red: TNFa with Cy3 labeled secondary antibody.
TNFa positive nerve fibers, mast cells, eosinophils
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 01 2013