Anti-TNF alpha抗体(ab34674)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: IP, RIA, ICC/IF, WB, Neutralising, IHC-P, ELISA
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TNF alpha抗体
参阅全部 TNF alpha 一抗 -
描述
兔多克隆抗体to TNF alpha -
宿主
Rabbit -
经测试应用
适用于: IP, RIA, ICC/IF, WB, Neutralising, IHC-P, ELISAmore details -
种属反应性
与反应: Mouse, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Mouse TNF alpha.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 26 kDa).
|
Neutralising |
Use at an assay dependent concentration.
It is recommended to incubate the sample with the dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. |
|
IHC-P | (2) |
Use at an assay dependent concentration.
|
ELISA |
Use at an assay dependent concentration.
For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. |
说明 |
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IP
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 26 kDa). |
Neutralising
Use at an assay dependent concentration. It is recommended to incubate the sample with the dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. |
靶标
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功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21926 Mouse
- SwissProt: P06804 Mouse
- Unigene: 1293 Mouse
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别名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
图片
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Anti-TNF alpha antibody (ab34674) at 1/1000 dilution + recombinant mouse TNF alpha, 50ng
Secondary
HRP rabbit secondary antibody at 1/40000 dilution
Predicted band size: 26 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (42)
ab34674 被引用在 42 文献中.
- Fu L et al. Dietary Supplement of Anoectochilus roxburghii (Wall.) Lindl. Polysaccharides Ameliorates Cognitive Dysfunction Induced by High Fat Diet via "Gut-Brain" Axis. Drug Des Devel Ther 16:1931-1945 (2022). PubMed: 35762015
- Aboulhoda BE et al. Histological, immunohistochemical, and molecular investigation on the hepatotoxic effect of potassium dichromate and the ameliorating role of Persea americana mill pulp extract. Microsc Res Tech 84:2434-2450 (2021). PubMed: 33908126
- Ertle CM et al. New Pathways for the Skin's Stress Response: The Cholinergic Neuropeptide SLURP-1 Can Activate Mast Cells and Alter Cytokine Production in Mice. Front Immunol 12:631881 (2021). PubMed: 33815383
- Abriz AE et al. Effect of docosahexaenoic acid plus insulin on atherosclerotic human endothelial cells. J Inflamm (Lond) 18:10 (2021). PubMed: 33602249
- Liu H et al. Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Nat Commun 12:114 (2021). PubMed: 33414464