Anti-TNF alpha抗体[NF-7] (ab9809)
Key features and details
- Mouse monoclonal [NF-7] to TNF alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-TNF alpha抗体[NF-7]
参阅全部 TNF alpha 一抗 -
描述
小鼠单克隆抗体[NF-7] to TNF alpha -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375 -
常规说明
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide -
Concentration information loading...
-
纯度
Protein A purified -
Primary antibody说明
This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells. -
克隆
单克隆 -
克隆编号
NF-7 -
骨髓瘤
NS0/1 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
-
Affibody® Molecule
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
靶标
-
功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 7124 Human
- Omim: 191160 Human
- SwissProt: P01375 Human
- Unigene: 241570 Human
-
别名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
图片
-
Overlay histogram showing THP1 cells stained with ab9809 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9809, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab9809 被引用在 2 文献中.
- Luna ACL et al. Modulation of pro-apoptotic effects and mitochondrial potential on B16F10 cells by DODAC/PHO-S liposomes. BMC Res Notes 11:126 (2018). PubMed: 29444697
- Hartley G et al. Regulation of PD-L1 expression on murine tumor-associated monocytes and macrophages by locally produced TNF-a. Cancer Immunol Immunother 66:523-535 (2017). PubMed: 28184968