Anti-TNF alpha抗体[MP6-XT22] - BSA and Azide free (ab34719)
Key features and details
- Rat monoclonal [MP6-XT22] to TNF alpha - BSA and Azide free
- Suitable for: ELISA, IHC-Fr, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TNF alpha抗体[MP6-XT22] - BSA and Azide free
参阅全部 TNF alpha 一抗 -
描述
大鼠单克隆抗体[MP6-XT22] to TNF alpha - BSA and Azide free -
宿主
Rat -
经测试应用
适用于: ELISA, IHC-Fr, Flow Cyt, Functional Studiesmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant full length protein corresponding to Mouse TNF alpha. Recombinant murine TNF alpha expressed in E. coli.
Database link: P06804 -
常规说明
This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
This antibody was purified from tissue culture supernatant. -
Primary antibody说明
This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha. -
克隆
单克隆 -
克隆编号
MP6-XT22 -
同种型
IgG1 -
研究领域
相关产品
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Affibody® Molecule
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34719于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilisation is required for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Functional Studies |
Use at an assay dependent concentration.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilisation is required for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. |
靶标
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功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21926 Mouse
- SwissProt: P06804 Mouse
- Unigene: 1293 Mouse
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别名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab34719 被引用在 7 文献中.
- Zhang Z et al. Design of a biofluid-absorbing bioactive sandwich-structured Zn-Si bioceramic composite wound dressing for hair follicle regeneration and skin burn wound healing. Bioact Mater 6:1910-1920 (2021). PubMed: 33364530
- Sommer K et al. 11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes. Int J Mol Sci 22:N/A (2021). PubMed: 34769092
- Kumstel S et al. MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases. Sci Rep 10:16931 (2020). PubMed: 33037288
- Brings S et al. Diabetes-induced alterations in tissue collagen and carboxymethyllysine in rat kidneys: Association with increased collagen-degrading proteinases and amelioration by Cu(II)-selective chelation. Biochim Biophys Acta 1852:1610-8 (2015). PubMed: 25900786
- Kimura N et al. Injury and subsequent regeneration of muscles for activation of local innate immunity to facilitate the development and relapse of autoimmune myositis in C57BL/6 mice. Arthritis Rheumatol 67:1107-16 (2015). IHC-Fr ; Mouse . PubMed: 25580817
- Jiménez AJ et al. Increased levels of tumour necrosis factor alpha (TNFα) but not transforming growth factor-beta 1 (TGFβ1) are associated with the severity of congenital hydrocephalus in the hyh mouse. Neuropathol Appl Neurobiol 40:911-32 (2014). PubMed: 24707814
- Scanga CA et al. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun 67:4531-8 (1999). PubMed: 10456896