Application
Western blot
Sample
Mouse Cell lysate - whole cell (CD14+)
Loading amount
20 µg
Specification
CD14+
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.6% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: lysis buffer
Secondary antibody
Name
Non-Abcam antibody was used: from LICOR
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ODISSEY SYSTEM (MMEDICAL) LECTURE 800NM
Exposure
1 second(s)
Bands
Specific: 55 kDa Non-specific: 100, 70, 65 kDa
Positive control
human CD14+
Negative control
none
Additional data
Additional Notes
1 human CD14+
2 mouse CD14+
2 mouse CD14+
Abcam response
We would like to thank this customer for their valuable feedback. This antibody was previously un-tested in mouse samples and the customer took part in our testing program and received a free of charge antibody as reward for testing ab15563 in mouse samples. We would like to hear from customer that used this antibody in mouse samples about their experiences.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 06 2012