Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
We use several antibodies to co-localize the interacting molecules including TNFR1. And the immaging core chose white for TNFR1.
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提交于 Jun 19 2014