Application
Flow Cytometry
Sample
Human Cell (Human immortalized microglial cell line)
Permeabilization
Yes - 1X Permeabilization buffer (eBioscience 00-8333-56)
Gating Strategy
A discrete population on the FSC-SSC scatter plot
Specification
Human immortalized microglial cell line
Preparation
Cell harvesting/tissue preparation method: Scraped cells from the plate
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 4°C
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 750
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 750
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 01 2018