重组Anti-TMEM119抗体[106-6] - Microglial marker (ab210405)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [106-6] to TMEM119 - Microglial marker
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
概述
-
产品名称
Anti-TMEM119抗体[106-6] - Microglial marker
参阅全部 TMEM119 一抗 -
描述
兔单克隆抗体[106-6] to TMEM119 - Microglial marker -
宿主
Rabbit -
特异性
Human reactivity has not been tested.
-
经测试应用
适用于: Flow Cytmore details
不适用于: IHC-Fr or IHC-P -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged mouse TMEM119.
-
常规说明
Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.
Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.
The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
106-6 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab210405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | (2) |
Use a concentration of 0.1 - 0.5 µg/ml.
For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474 |
说明 |
---|
Flow Cyt
Use a concentration of 0.1 - 0.5 µg/ml. For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474 |
靶标
-
细胞定位
Membrane; Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 231633 Mouse
- SwissProt: Q8R138 Mouse
-
别名
- OBIF antibody
- Osteoblast induction factor antibody
- PSEC0199 antibody
see all
图片
-
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged mouse TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.
Alexa Fluor® 488 (ab225497) and R-PE (ab225496) conjugated versions are available for this clone.
-
Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).
The data was provided by Ben Barres’ lab (Stanford University).
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (16)
ab210405 被引用在 16 文献中.
- Uff CEG et al. Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue. Biomolecules 12:N/A (2022). PubMed: 35625531
- Rong K et al. Alendronate Alleviated Femoral Head Necrosis and Upregulated BMP2/EIF2AK3/EIF2A/ATF4 Pathway in Liquid Nitrogen Treated Rats. Drug Des Devel Ther 15:1717-1724 (2021). PubMed: 33935494
- Deerhake ME et al. Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M. Immunity 54:484-498.e8 (2021). PubMed: 33581044
- Ochocka N et al. Single-cell RNA sequencing reveals functional heterogeneity of glioma-associated brain macrophages. Nat Commun 12:1151 (2021). PubMed: 33608526
- Olmsted-Davis E et al. A Population of M2 Macrophages Associated With Bone Formation. Front Immunol 12:686769 (2021). PubMed: 34712222