Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate cell line LNCaP)
Permeabilization
No
Specification
Prostate cell line LNCaP
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: Blockin buffer
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti Rabbit FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
The antibody was used to detect FAM3C on LNCaP cell lines. The negative control was one with only the secondary antibody incubation and it showed no staining at all.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 10 2012