生物素Anti-TLR9抗体[5G5] (ab17236)
Key features and details
- Biotin Mouse monoclonal [5G5] to TLR9
- Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Dog, Human
- Conjugation: Biotin
- Isotype: IgG2a
概述
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产品名称
生物素Anti-TLR9抗体[5G5]
参阅全部 TLR9 一抗 -
描述
生物素小鼠单克隆抗体[5G5] to TLR9 -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Dog, Human -
免疫原
Recombinant fragment within TLR9. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- RAW macrophages and TLR9 transfected HEK293 cells. RAW264.7 macrophages stimulated with IFN gamma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯化说明
0.2 µm filtered -
克隆
单克隆 -
克隆编号
5G5 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17236于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
细胞定位
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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数据库链接
- Entrez Gene: 54106 Human
- Entrez Gene: 81897 Mouse
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- SwissProt: Q9EQU3 Mouse
- Unigene: 87968 Human
- Unigene: 44889 Mouse
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别名
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab17236 被引用在 5 文献中.
- Wang CY et al. TLR9 Binding to Beclin 1 and Mitochondrial SIRT3 by a Sodium-Glucose Co-Transporter 2 Inhibitor Protects the Heart from Doxorubicin Toxicity. Biology (Basel) 9:N/A (2020). PubMed: 33138323
- Liu FY et al. TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis. Cell Death Dis 10:480 (2019). PubMed: 31209243
- Chen GY et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). ICC/IF, Flow Cyt ; Mouse . PubMed: 22704844
- Chen GY et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). Flow Cyt ; Human . PubMed: 19656899
- Tomchuck SL et al. Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. Stem Cells 26:99-107 (2008). Flow Cyt ; Human . PubMed: 17916800