生物素Anti-TLR4抗体[MTS510] (ab9105)
Key features and details
- Biotin Rat monoclonal [MTS510] to TLR4
- Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
概述
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产品名称
生物素Anti-TLR4抗体[MTS510]
参阅全部 TLR4 一抗 -
描述
生物素大鼠单克隆抗体[MTS510] to TLR4 -
宿主
Rat -
偶联物
Biotin -
特异性
Only detects murine TLR-4 when complexed to MD-2. This antibody has been found to coprecipitate MD-2 (30kDa) with TLR4 (100kDa). -
经测试应用
适用于: IHC-Fr, IP, Flow Cyt, Functional Studiesmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to TLR4. Mouse Ba/F3 cells expressing mouse TLR4
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常规说明
Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
0.2 µm filtered -
Primary antibody说明
Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells. -
克隆
单克隆 -
克隆编号
MTS510 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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Functional Studies |
Use at an assay dependent concentration.
|
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. |
靶标
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功能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
组织特异性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
疾病相关
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
结构域
The TIR domain mediates interaction with NOX4. -
翻译后修饰
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21898 Mouse
- SwissProt: Q9QUK6 Mouse
- Unigene: 38049 Mouse
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别名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab9105 被引用在 2 文献中.
- Chakraborty D et al. Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner. Front Immunol 8:1493 (2017). PubMed: 29180999
- Daubeuf B et al. TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. J Immunol 179:6107-14 (2007). Flow Cyt ; Mouse . PubMed: 17947685