Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3 fibroblast, N2a neuronal cell, primary macr)
Loading amount
0.2 µg
Specification
NIH3T3 fibroblast, N2a neuronal cell, primary macr
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Block solution (5% milk)
Secondary antibody
Name
Non-Abcam antibody was used: GE Healthcare. NA931V ECL Anti-mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Sheep
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
Millipore, Immobilon Western
Exposure
4 second(s)
Bands
Specific: 120 for N2a kDa Non-specific: 250 kDa
Additional data
Additional Notes
Lane 1 and 2: NIH3T3 mouse fibroblast cell line
Lane 3: N2a mouse neuronal cell line
Lane 4: Mouse primary bone marrow macrophage
Lane 5: Same as 4
Lane 6: Mouse primary bone marrow macrophage. Sonicated after cell lysis.
Lane 3: N2a mouse neuronal cell line
Lane 4: Mouse primary bone marrow macrophage
Lane 5: Same as 4
Lane 6: Mouse primary bone marrow macrophage. Sonicated after cell lysis.
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Mikael Rutberg
Verified customer
提交于 Sep 05 2012