Anti-TLR3抗体[TLR3.7] (ab12085)
Key features and details
- Mouse monoclonal [TLR3.7] to TLR3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-TLR3抗体[TLR3.7]
参阅全部 TLR3 一抗 -
描述
小鼠单克隆抗体[TLR3.7] to TLR3 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra)more details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein (DDDDK-tag) corresponding to Human TLR3. Human DDDDK-tagged TLR3 stably expressed by Ba/F3 cells.
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阳性对照
- Placenta and pancreas. Monocyte-derived immature dendritic cells (see paper by Matsumoto et al,2003). Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells Flow Cyt (Intra):THP-1 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
0.2 µm filtered -
克隆
单克隆 -
克隆编号
TLR3.7 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12085于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10.
See paper by Matsumoto et al, 2003.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody |
说明 |
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Flow Cyt (Intra)
1/10. See paper by Matsumoto et al, 2003.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
疾病相关
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
结构域
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
翻译后修饰
Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
细胞定位
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7098 Human
- Omim: 603029 Human
- SwissProt: O15455 Human
- Unigene: 657724 Human
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别名
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
图片
实验方案
数据表及文件
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Datasheet download
文献 (12)
ab12085 被引用在 12 文献中.
- Bhagat S et al. Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling. Blood Cells Mol Dis 84:102459 (2020). PubMed: 32559654
- Ishida Y et al. Candida albicans ß-Glucan-Containing Particles Increase HO-1 Expression in Oral Keratinocytes via a Reactive Oxygen Species/p38 Mitogen-Activated Protein Kinase/Nrf2 Pathway. Infect Immun 86:N/A (2018). PubMed: 29311246
- Hwang SH et al. Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor. PLoS One 10:e0138041 (2015). PubMed: 26376485
- Yuan MM et al. TLR3 expression correlates with apoptosis, proliferation and angiogenesis in hepatocellular carcinoma and predicts prognosis. BMC Cancer 15:245 (2015). IHC . PubMed: 25884709
- Biswas I et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). PubMed: 26350442