Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary macrophage)
Specification
Primary macrophage
Fixative
Paraformaldehyde
Permeabilization
Yes - Acetone, 5 min, -20
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/150
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: anti-rat IgG - Cy5
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy5 ®
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy5 ®
Dilution
1/500
Additional data
Additional Notes
Used on primary macrophages expressing endogenous levels of TLR2. Also works on other cell types expressing lower levels of TLR2 (e.g. primary epithelia) but requires more antibody (1:50, overnight at 4 degrees).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 26 2013