Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP-1)
Specification
THP-1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% goat serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa-Fluor 488 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
PMA-treated THP-1 cells stained with Mal (secondary stained with Alexa-Fuor 488 goat anti-rabbit - green) and Alexa Fluor 595-conjugated wheat germ agglutinin (red). Nuclei were stained with Hoechst 33258. Cells were fixed for 30 min at room temperature.
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提交于 Aug 10 2011