Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Uterus/ Ovary/ Fallopian tube)
Permeabilization
Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification
Uterus/ Ovary/ Fallopian tube
Blocking step
3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA / 3% donkey serum in PBST
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Image shows frozen section of adult mouse fallopian tube. We detected reliably positive TIMP3 staining in the extracellular space. The image shows DAPI counterstain, red field (autofluorescence), green field antibody staining and overlay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Julia Watson
Verified customer
提交于 Jun 03 2015