Anti-TIMP1抗体- Carboxyterminal end (ab38978)

概述

  • 产品名称
    Anti-TIMP1抗体- Carboxyterminal end
    参阅全部 TIMP1 一抗
  • 描述
    兔多克隆抗体to TIMP1 - Carboxyterminal end
  • 特异性
    This antibody recognizes both reduced and non reduced TIMP1 protein, but does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4).
  • 经测试应用
    适用于: ELISA, IP, WB, Sandwich ELISA, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide based on the carboxyterminal region of human TIMP1.

  • 阳性对照
    • WB: Human TIMP1 and cell media from human chondrosarcoma cells treated with TPA.

性能

应用

Our Abpromise guarantee covers the use of ab38978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/1000 - 1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 29 kDa when used against the reduced protein. Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab28261 as Capture.
ICC Use at an assay dependent concentration.

靶标

  • 功能
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • 序列相似性
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻译后修饰
    The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clgi antibody
    • Collagenase inhibitor antibody
    • Collagenase inhibitor, Human antibody
    • EPA antibody
    • EPO antibody
    • Erythroid Potentiating Activity antibody
    • Erythroid-potentiating activity antibody
    • Fibroblast collagenase inhibitor antibody
    • FLJ90373 antibody
    • HCI antibody
    • Human Collagenase Inhibitor antibody
    • Metalloproteinase inhibitor 1 antibody
    • Metalloproteinase inhibitor 1 precursor antibody
    • OTTHUMP00000023214 antibody
    • TIMP 1 antibody
    • TIMP antibody
    • TIMP metallopeptidase inhibitor 1 antibody
    • TIMP-1 antibody
    • Timp1 antibody
    • TIMP1 protein antibody
    • TIMP1_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 1 antibody
    • Tissue inhibitor of metalloproteinases 1 antibody
    • Tissue inhibitor of metalloproteinases antibody
    • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
    see all

图片

  • All lanes : Anti-TIMP1 antibody - Carboxyterminal end (ab38978) at 1/1000 dilution

    Lane 1 : Human TIMP1
    Lane 2 : Cell media from human chondrosarcoma (no treatment)
    Lane 3 : Cell media from human chondrosarcoma (treated with TPA)


    Predicted band size : 23 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.


  • Predicted band size : 23 kDa
    Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.

    Image courtesy of Koji Ueda by Abreview

    Each lane corresponds to a distinct lung adenocarcinoma patient sample. The primary antibody was used at 1/3000 dilution. 10µg/ml lysate has been added per lane. A sheep anti-rabbit monoclonal conjugated to HRP was used as the secondary antibody at 1/10,000 dilution.

    Detection method used was Western Lightning. 3 minutes. Performed under reducing conditions.

    Samples blocked using 5% BSA for 1 hour at 25°C.
    Non-specific band at 27kDa is thought to be Ig light chain.

    See Abreview

文献

This product has been referenced in:
  • Zhang J  et al. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem 398:123-34 (2015). Read more (PubMed: 25200140) »
  • Urso ML  et al. Adenosine A3 receptor stimulation reduces muscle injury following physical trauma and is associated with alterations in the MMP/TIMP response. J Appl Physiol 112:658-70 (2012). WB ; Mouse . Read more (PubMed: 22114177) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (endothelial cell)
Specification
endothelial cell
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X 0.1%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial cell)
Loading amount
5000 cells
Specification
endothelial cell
Treatment
Adenoviral overexpression
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Feb 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (human serum)
Loading amount
10 µg
Specification
human serum
Treatment
MARS Hu-14 column (Agilent)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

幸嗣 植田

Verified customer

提交于 May 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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