Application
Western blot
Sample
Human Cell lysate - whole cell (various incl: HeLa, HEK293, 786-O, MCF-7)
Loading amount
30 µg
Specification
various incl: HeLa, HEK293, 786-O, MCF-7
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/250
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Milk, 0.1% Tween-20, PBS
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal West Dura
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 30 kDa Non-specific: ~120 (faint) kDa
Positive control
HeLa
Additional data
Additional Notes
Lane order (L-R): HeLa, HEK293, 786-O, MCF-7
Detects a species of the predicted mobility in all lysates tested (30 kDa).
Detects a species of the predicted mobility in all lysates tested (30 kDa).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Oct 07 2008