Anti-TICAM2抗体[mAbcam77169] (ab77169)
Key features and details
- Mouse monoclonal [mAbcam77169] to TICAM2
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-TICAM2抗体[mAbcam77169]
参阅全部 TICAM2 一抗 -
描述
小鼠单克隆抗体[mAbcam77169] to TICAM2 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TICAM2 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab57980) -
阳性对照
- Recombinant Human TICAM2 protein (ab123160) can be used as a positive control in WB. This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
mAbcam77169 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77169于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. -
组织特异性
Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas. -
序列相似性
Contains 1 TIR domain. -
结构域
The TIR domain mediates the interaction with TRAF6. -
翻译后修饰
Phosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling. -
细胞定位
Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 100302736 Human
- Entrez Gene: 353376 Human
- Omim: 608321 Human
- SwissProt: Q86XR7 Human
- Unigene: 709120 Human
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别名
- Putative NF-kappa-B-activating protein 502 antibody
- TCAM2_HUMAN antibody
- TICAM-2 antibody
see all
图片
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All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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Datasheet download
文献 (0)
ab77169 尚未被引用在任何文献中。