Application
Flow Cytometry
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.25 % Triton X-100
Gating Strategy
Viable cells
Specification
Brain
Fixation
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+5% normal donkey serum
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
1 mL of 0.25 % Triton X-100 in PBS buffer was added to cells pellets in order to permeablize cells membrane. The samples were incubated for 30 minutes at room temperature with rocking, protected from light and washed once with 1 mL of PBS. Next, the cells were resuspended in blocking buffer containing 5% normal donkey serum in PBS and incubated for 2 hrs at room temperature with rocking, protected from light. The cell suspensions were stained overnight at 4 ¯C temperature with primary rabbit polyclonal TGF beta R1-phospho (1:100) protected from light with rocking.
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in rat, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in rat which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Aneta Radziwon-Balicka
Verified customer
提交于 Jan 18 2018