Anti-TGF beta 1抗体(ab92486)
Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TGF beta 1抗体
参阅全部 TGF beta 1 一抗 -
描述
兔多克隆抗体to TGF beta 1 -
宿主
Rabbit -
特异性
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
ab92486 detects the mature secreted form of the protein at 13 kDa. Our internal testing data suggests that this does not detect the full length protein at 44kDa. As cell lysates are unsuitable for detection of the mature form, tissue lysates should be used when using this antibody.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.
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阳性对照
- WB: Human spleen and kidney tissue lysates.
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 5559 Anti-TGF-ß1 Polyclonal Antibody. 5559-100 is the same size as the 100 µg size of ab92486.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), 0.15% EDTA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa.
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
说明 |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
靶标
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功能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
组织特异性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
疾病相关
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
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别名
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
图片
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All lanes : Anti-TGF beta 1 antibody (ab92486)
Lane 1 : Human recombinant TGF-ß1 at 0.0005 µg
Lane 2 : Human kidney tissue at 50 µg
Lane 3 : Human spleen tissue at 50 µg
Predicted band size: 44 kDa -
All lanes : Anti-TGF beta 1 antibody (ab92486) at 5 µg/ml
Lane 1 : Human spleen tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab92486 observed at 15 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (596)
ab92486 被引用在 596 文献中.
- Yamane S et al. The role of thromboxane prostanoid receptor signaling in gastric ulcer healing. Int J Exp Pathol 103:4-12 (2022). PubMed: 34655121
- Bi L et al. Pirfenidone Attenuates Renal Tubulointerstitial Fibrosis through Inhibiting miR-21. Nephron 146:110-120 (2022). PubMed: 34724669
- Wu J et al. Rubus chingii Hu. unripe fruits extract ameliorates carbon tetrachloride-induced liver fibrosis and improves the associated gut microbiota imbalance. Chin Med 17:56 (2022). PubMed: 35549741
- Mancinelli R et al. The Effects of Taurocholic Acid on Biliary Damage and Liver Fibrosis Are Mediated by Calcitonin-Gene-Related Peptide Signaling. Cells 11:N/A (2022). PubMed: 35563897
- Wang J et al. Empagliflozin Ameliorates Diabetic Cardiomyopathy via Attenuating Oxidative Stress and Improving Mitochondrial Function. Oxid Med Cell Longev 2022:1122494 (2022). PubMed: 35585884