Anti-TGF alpha抗体(ab9585)
Key features and details
- Rabbit polyclonal to TGF alpha
- Suitable for: ELISA, Neutralising, Sandwich ELISA, WB
- Reacts with: Mouse, Human
- Isotype: unknown
概述
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产品名称
Anti-TGF alpha抗体
参阅全部 TGF alpha 一抗 -
描述
兔多克隆抗体to TGF alpha -
宿主
Rabbit -
特异性
The immunogen shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. -
经测试应用
适用于: ELISA, Neutralising, Sandwich ELISA, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein aa 1-100.
Database link: P01135 -
常规说明
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab208156
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C. -
存放说明
Shipped at 4°C. Store at -20ºC. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required. |
|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α. |
|
WB |
Use at an assay dependent concentration.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required. |
Sandwich ELISA
Use at an assay dependent concentration. To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α. |
WB
Use at an assay dependent concentration. |
靶标
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功能
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
组织特异性
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
序列相似性
Contains 1 EGF-like domain. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 7039 Human
- Entrez Gene: 21802 Mouse
- Omim: 190170 Human
- SwissProt: P01135 Human
- SwissProt: P48030 Mouse
- Unigene: 170009 Human
- Unigene: 137222 Mouse
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别名
- EGF like TGF antibody
- EGF-like TGF antibody
- ETGF antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (17)
ab9585 被引用在 17 文献中.
- Wei Y et al. EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression. J Bone Miner Res 37:1012-1023 (2022). PubMed: 35191092
- Tofteng SS et al. Increased COX-2 after ureter obstruction attenuates fibrosis and is associated with EP2 receptor upregulation in mouse and human kidney. Acta Physiol (Oxf) 235:e13828 (2022). PubMed: 35543087
- Cai W et al. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice. J Clin Invest 132:N/A (2022). PubMed: 35912857
- Bunker EN et al. Suppression of a-catenin and adherens junctions enhances epithelial cell proliferation and motility via TACE-mediated TGF-a autocrine/paracrine signaling. Mol Biol Cell 32:348-361 (2021). PubMed: 33378218
- Nishii K et al. Triple therapy with osimertinib, bevacizumab and cetuximab in EGFR-mutant lung cancer with HIF-1a/TGF-a expression. Oncol Lett 22:639 (2021). PubMed: 34386061