Anti-TEM4抗体(ab67278)
Key features and details
- Rabbit polyclonal to TEM4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TEM4抗体 -
描述
兔多克隆抗体to TEM4 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat cell lysate. Human spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 222 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 222 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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相关性
TEM4 (Tumor endothelial marker 4) is a Rho-specific GEF (guanine-nucleotide exchange factor) with novel structural and regulatory properties and predominant expression in the heart. Rho GTPases, which are activated by specific GEFs, play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful as a target, therapeutically. -
数据库链接
- Entrez Gene: 9828 Human
- SwissProt: Q96PE2 Human
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别名
- 164 kDa Rho specific guanine nucleotide exchange factor antibody
- ARHGEF 17 antibody
- ARHGEF17 antibody
see all
图片
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Lane 1 : Anti-TEM4 antibody (ab67278) at 1 µg/ml
Lane 2 : Anti-TEM4 antibody (ab67278) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 222 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted? -
ab67278 at 2.5 μg/ml staining of TEM4 in human spleen tissue using IHC-P.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab67278 被引用在 2 文献中.
- Prifti DK et al. A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA). PLoS One 17:e0268848 (2022). PubMed: 35776709
- Isokane M et al. ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores. J Cell Biol 212:647-59 (2016). WB . PubMed: 26953350