Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis (human mouse and rat))
Specification
Testis (human mouse and rat)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit polymer HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Did not produce nuclear staining and no primary control was clean. Will repeat with different antigen retrieval.
Abcam response
Thank you for your Abreview. This antibody has not previsouly been tested in IHC. As you suggest, repeating with a different antigen retrieval technique may yield more conclusive results. Good luck with your experiment.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Jun 04 2008