Anti-TBLR1/TBL1XR1抗体[4F3-A8-D9] (ab117761)
Key features and details
- Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
概述
-
产品名称
Anti-TBLR1/TBL1XR1抗体[4F3-A8-D9]
参阅全部 TBLR1/TBL1XR1 一抗 -
描述
小鼠单克隆抗体[4F3-A8-D9] to TBLR1/TBL1XR1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, African green monkey -
免疫原
Recombinant fragment corresponding to Human TBLR1/TBL1XR1.
Database link: Q9BZK7 -
阳性对照
- WB: K562 whole cell lysate, Mouse heart lysate, Mouse brain lysate ICC: HeLa cells IHC-P: Human breast cancer
-
常规说明
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
-
纯度
Concentrated Culture Supernatant -
克隆
单克隆 -
克隆编号
4F3-A8-D9 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
|
|
IHC-P |
1/500 - 1/1000.
|
|
ICC/IF |
1/150 - 1/200.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). |
IHC-P
1/500 - 1/1000. |
ICC/IF
1/150 - 1/200. |
靶标
-
功能
F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats. -
结构域
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 79718 Human
- Entrez Gene: 81004 Mouse
- Omim: 608628 Human
- SwissProt: Q9BZK7 Human
- SwissProt: Q8BHJ5 Mouse
- Unigene: 581171 Human
- Unigene: 202966 Mouse
- Unigene: 446265 Mouse
-
别名
- C21 antibody
- DC42 antibody
- F box like/WD repeat containing protein TBL1XR1 antibody
see all
图片
-
ab117761, at 1/150 dilution, staining TBLR1/TBL1XR1 in HeLa cells by Immunocytochemistry. This image was produced with antibody produced in ascites.
-
All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 2 : CEM cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 56 kDa -
All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Heart Lysate
Lane 3 : K562 cell Lysate
Predicted band size: 56 kDaThis image was produced with antibody produced in ascites.
-
ab117761, at 1/500 - 1/1000 dilution, staining TBLR1/TBL1XR1 in paraffin-embedded Human breast cancer by Imunohistochemistry. This image was produced with antibody produced in ascites.
-
Immunocytochemical staining of HeLa cells using anti-TBLR1/TBL1XR1 antibody (ab117761) at a dilution of 1:200. Cells were fixed with 4% paraformaldehyde. This image was produced with antibody produced in ascites.
-
Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution + K562 whole cell lysate at 40 µg
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
-
All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab117761 被引用在 2 文献中.
- Zhou Q et al. Transducin (ß)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway. Oncogene 36:1873-1886 (2017). WB, IF . PubMed: 27694893
- Ma M & Yu N Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer. Tohoku J Exp Med 241:239-247 (2017). PubMed: 28344213