Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Loading amount
50000 cells
Specification
MDA-MB-231 breast cancer cell line
Gel Running Conditions
Reduced Denaturing (10% PAGE)
Blocking step
2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 20% blocking solution in PBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 44 kDa Non-specific: 60, 45, 28, 7 kDa
Additional data
Additional Notes
This antibody shows a dominant band of the expected size. Some faint aspecific bands can be observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 28 2012