Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - whole (0-4hr embryos)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Treatment
control shRNA and Tao shRNA embryos
Specification
0-4hr embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk/TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Amersham Anti-Rabbit IgG, peroxidase-linked whole
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce Femto ECL
Negative control
0-4hr old Tao shRNA embryos (lane 2), determined by qPCR to achieve 91% knockdown of Tao and exhibit phenotype
Exposure
10 second(s)
Bands
Specific: 180, 150, 105, 60 kDa Non-specific: 130, 32 kDa
Positive control
0-4hr old EGFP shRNA embryos (lane 1)
Additional data
Abcam response
Many thanks for your valuable feedback. It appears that under these experimental conditions, ab32591 does not specifically detect TAO kinase 2 (phospho S181) in Drosophila melanogaster. We welcome further customer comments. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Richelle Sopko
Verified customer
提交于 Mar 04 2014