Anti-Syntenin抗体(ab19903)
Key features and details
- Rabbit polyclonal to Syntenin
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Syntenin抗体
参阅全部 Syntenin 一抗 -
描述
兔多克隆抗体to Syntenin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Xenopus laevis, Monkey, Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
靶标
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功能
Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway. -
组织特异性
Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta. -
序列相似性
Contains 2 PDZ (DHR) domains. -
翻译后修饰
Phosphorylated on tyrosine residues. -
细胞定位
Cell junction > focal adhesion. Cell junction > adherens junction. Cell membrane. Endoplasmic reticulum membrane. Nucleus. Melanosome. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 6386 Human
- Entrez Gene: 53378 Mouse
- Entrez Gene: 83841 Rat
- Entrez Gene: 447442 Xenopus laevis
- Entrez Gene: 325004 Zebrafish
- Omim: 602217 Human
- SwissProt: O00560 Human
- SwissProt: O08992 Mouse
see all -
别名
- MDA-9 antibody
- MDA9 antibody
- Melanoma differentiation-associated protein 9 antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Syntenin knockout HAP1 whole cell lysate (20 µg)
Lane 3: A549 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab19903 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab19903 was shown to recognize Syntenin in wild-type HAP1 cells as signal was lost at the expected MW in Syntenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Syntenin knockout samples were subjected to SDS-PAGE. ab19903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Syntenin antibody (ab19903) at 1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : Human heart tissue lysate
Lane 3 : HEK-293 cell lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lane 4 : Human heart tissue lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDaab19903 detects a band of the expected size (32kDa) in HEK-293 cell lysate. This band is partially quenched by the addition of ab20431. No band is observed in human heart lysate.
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All lanes : Anti-Syntenin antibody (ab19903) at 1 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lane 4 : Rat heart lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
ab19903 detects a band of the expected size (32kDa) in both mouse and rat heart lysate. This band is quenched completely by the addition of the immunizing peptide in the mouse lysate and is partially quenched by the addition of ab20431 in the rat heart lysate. -
ICC/IF image of ab19903 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab19903, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab19903 被引用在 36 文献中.
- Ma L et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair. Bioact Mater 19:626-641 (2023). PubMed: 35600968
- Njock MS et al. Endothelial extracellular vesicles promote tumour growth by tumour-associated macrophage reprogramming. J Extracell Vesicles 11:e12228 (2022). PubMed: 35656866
- Kang Y et al. Serum and Serum Exosomal CircRNAs hsa_circ_0001492, hsa_circ_0001439, and hsa_circ_0000896 as Diagnostic Biomarkers for Lung Adenocarcinoma. Front Oncol 12:912246 (2022). PubMed: 35747792
- Costa A et al. Investigating the Paracrine Role of Perinatal Derivatives: Human Amniotic Fluid Stem Cell-Extracellular Vesicles Show Promising Transient Potential for Cardiomyocyte Renewal. Front Bioeng Biotechnol 10:902038 (2022). PubMed: 35757808
- Malkin EZ et al. Cell-free DNA topology depends on its subcellular and cellular origins in cancer. JCI Insight 7:N/A (2022). PubMed: 36125881