Application
Western blot
Sample
Human Cell lysate - whole cell (Several cell lines)
Gel Running Conditions
Reduced Denaturing (10 %)
Loading amount
30 µg
Specification
Several cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 31 kDa
Additional data
Additional Notes
Lanes
1 - 789-0 Kidneyadenoma Human
2 - HSC 2/8 Chondosarcoma Human
3 - HT-29 Darm Human
4 - LS174T Intestine Human
5 - MCF7 Breast Human
6 - Sk-Mel-28 Melanoma Human
7 - SW620 Intestine Human
8 - H35 Liver Rat
Beta actin included
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Monika Schumacher
Verified customer
提交于 Jan 16 2015