Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (IPS-derived Neurons)
Specification
IPS-derived Neurons
Blocking step
5% GS as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Methanol
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% GS
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Life Technologies
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
Obtained the ab from abcam - a 10 ul aliquot!!! used once (1 ul) and when wanted use after 10 days, nothing remained in the tube!!! And the staining was negative!!!
Abcam response
Thank you for your valuable feedback. We are sorry to hear that product did not work as expected in this occasion. It could be a vial/lot specific problem that we were unaware of. We hope that the replacement product that you requested will give you good results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 27 2017