重组Anti-Synapsin I (phospho S9)抗体[EP2162Y] (ab76260)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Synapsin I (phospho S9)抗体[EP2162Y]
参阅全部 Synapsin I 一抗 -
描述
兔单克隆抗体[EP2162Y] to Synapsin I (phospho S9) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human, mouse and rat brain tissue lysates, untreated or treated with AP.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2162Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76260于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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Dot blot |
1/1000.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
Dot blot
1/1000. |
靶标
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功能
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
疾病相关
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
序列相似性
Belongs to the synapsin family. -
翻译后修饰
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
别名
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
图片
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution
Lanes 1 & 3 : Human brain whole cell lysate - untreated
Lanes 2 & 4 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?Exposure time:
Lanes 1 and 2: 1 second.
Lanes 3 and 4: 15 seconds.
Blocking and dilution buffer:
Lanes 1 and 2: 5% NFDM/TBST.
Lanes 3 and 4: 2% BSA/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution
Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution
Lane 1 : Mouse brain whole cell lysate - untreated
Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution
Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and dilution buffer: 2% BSA/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)
Lane 1 : Human brain tissue lysate, untreated
Lane 2 : Human brain tissue lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab76260 被引用在 6 文献中.
- Shi HJ et al. Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine. Theranostics 12:3656-3675 (2022). PubMed: 35664081
- Yan P et al. Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons. Cell Death Dis 13:786 (2022). PubMed: 36097267
- Dou Y et al. Neurotransmitter-stimulated neuron-derived sEVs have opposite effects on amyloid β-induced neuronal damage. J Nanobiotechnology 19:324 (2021). PubMed: 34654438
- Campos CA & Ritter RC NMDA-type glutamate receptors participate in reduction of food intake following hindbrain melanocortin receptor activation. Am J Physiol Regul Integr Comp Physiol 308:R1-9 (2015). PubMed: 25394828
- Campos CA et al. Central vagal afferent endings mediate reduction of food intake by melanocortin-3/4 receptor agonist. J Neurosci 34:12636-45 (2014). IHC-FoFr ; Rat . PubMed: 25232103
- Semerdjieva S et al. Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors. Mol Cell Biol 33:1442-55 (2013). PubMed: 23382070