Anti-Surfactant蛋白A/PSAP抗体(ab87674)
Key features and details
- Rabbit polyclonal to Surfactant Protein A/PSAP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Surfactant蛋白A/PSAP抗体
参阅全部 Surfactant Protein A/PSAP 一抗 -
描述
兔多克隆抗体to Surfactant蛋白A/PSAP -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Pig, Non human primates -
免疫原
Synthetic peptide corresponding to Human Surfactant Protein A/PSAP aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab87829) -
阳性对照
- This antibody gave a positive signal in Human Lung Tissue Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human Lung.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab87674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa). |
IHC-P
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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细胞定位
Secreted protein; extracellular space. -
数据库链接
- Entrez Gene: 6435 Human
- Entrez Gene: 6436 Human
- Entrez Gene: 397503 Pig
- Omim: 178630 Human
- Omim: 178642 Human
- SwissProt: Q8IWL1 Human
- SwissProt: Q8IWL2 Human
- SwissProt: P49874 Pig
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别名
- 35 kDa pulmonary surfactant-associated protein antibody
- Alveolar proteinosis protein antibody
- COLEC4 antibody
see all
图片
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IHC image of Protein Surfactant A/PSAP staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab87674, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Anti-Surfactant Protein A/PSAP antibody (ab87674) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesSurfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab87674 被引用在 1 文献中.
- Stoeckelhuber M et al. Immunolocalization of Surfactant Proteins SP-A, SP-B, SP-C, and SP-D in Infantile Labial Glands and Mucosa. J Histochem Cytochem 66:531-538 (2018). PubMed: 29601229