Anti-Surf1抗体[21H2BG4] (ab110256)
Key features and details
- Mouse monoclonal [21H2BG4] to Surf1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Surf1抗体[21H2BG4]
参阅全部 Surf1 一抗 -
描述
小鼠单克隆抗体[21H2BG4] to Surf1 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯化说明
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
21H2BG4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110256于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
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Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Probably involved in the biogenesis of the COX complex. -
疾病相关
Defects in SURF1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency. -
序列相似性
Belongs to the SURF1 family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6834 Human
- Omim: 185620 Human
- SwissProt: Q15526 Human
- Unigene: 512464 Human
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别名
- OTTHUMP00000022473 antibody
- OTTHUMP00000022474 antibody
- SURF 1 antibody
see all
图片
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Overlay histogram showing HepG2 cells stained with ab110256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110256, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Complex IV assembly factor SURF1 identification. This mAb can be used to verify the presence of Complex IV assembly factor SURF1. Blotted alongside are mAbs against the ATP synthase subunit F1α subunit, an abundant mitochondrial protein acting as a control for gel loading. To indicate the assembly state of the cytochrome c oxidase, mAbs against the nuclear cytochrome c oxidase subunit COXVα and the mitochondrially encoded COXII subunit are also blotted in this Figure.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab110256 被引用在 4 文献中.
- Echaniz-Laguna A et al. SURF1 deficiency causes demyelinating Charcot-Marie-Tooth disease. Neurology 81:1523-30 (2013). WB ; Human . PubMed: 24027061
- Lopes Costa A et al. Beneficial effects of resveratrol on respiratory chain defects in patients' fibroblasts involve estrogen receptor and estrogen-related receptor alpha signaling. Hum Mol Genet N/A:N/A (2013). PubMed: 24365713
- Bastin J et al. Activation of peroxisome proliferator-activated receptor pathway stimulates the mitochondrial respiratory chain and can correct deficiencies in patients' cells lacking its components. J Clin Endocrinol Metab 93:1433-41 (2008). PubMed: 18211970
- Dell'agnello C et al. Increased longevity and refractoriness to Ca(2+)-dependent neurodegeneration in Surf1 knockout mice. Hum Mol Genet 16:431-44 (2007). WB ; Mouse . PubMed: 17210671