Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, SKOV-3, CaOV-3)
Loading amount
15 µg
Specification
HEK293, SKOV-3, CaOV-3
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Aqua Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Dilution
1/300
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Aqua Block
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
Li-Cor Fluorescent Reader
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~50 kDa Non-specific: Cell Line Specific - kDa
Additional data
Additional Notes
RIPA buffer with protease and phosphatase inhibitors
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 07 2011