Anti-Superoxide Dismutase 3/EC-SOD抗体(ab90258)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Superoxide Dismutase 3/EC-SOD抗体
参阅全部 Superoxide Dismutase 3/EC-SOD 一抗 -
描述
兔多克隆抗体to Superoxide Dismutase 3/EC-SOD -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following human tissue lysates: Artery; Heart; Lung; Kidney.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab90258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
组织特异性
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
序列相似性
Belongs to the Cu-Zn superoxide dismutase family. -
细胞定位
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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数据库链接
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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别名
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
图片
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All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258) at 1 µg/ml
Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Lung (Human) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32,35 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 42 kDa, 50 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesSuperoxide dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt), which may explain the migration pattern observed.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab90258 被引用在 1 文献中.
- Katamune C et al. Mutation of the gene encoding the circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulating the Aldh3a1 gene. J Biol Chem 294:547-558 (2019). PubMed: 30429219