Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain 20um thick)
Permeabilization
Yes - 0.1% triton-x
Specification
Brain 20um thick
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% tx with 10% NGS in PBS
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti-rb alexa 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
speckling of background seems unavoidable and consistent with other review images here. However it does show great staining in the cytoplasm and nuclei of certain models under stress.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kelly Maers
Verified customer
提交于 Jan 24 2017