Application
Western blot
Sample
Plants Tissue lysate - whole (anther)
Loading amount
30 µg
Specification
anther
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 1xTBST
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-rabbit-IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 22, 35, 40, 45, 66, kDa
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 06 2012