Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
1000 µg
Specification
HeLa
Immuno-precipitation step
Protein G
Other product details
Concentration
4 µg/mg lysate
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NTEN Buffer
Western Blot antibody
Name
Abcam antibody:Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (TR)
Dilution
1/1000
Additional data
Additional Notes
Lane 1: 2% of the total input
Lane 2: Ip with control IgG
Lane 3: Ip with specific antibody anti-STIM1 (ab57834)
Lane 2: Ip with control IgG
Lane 3: Ip with specific antibody anti-STIM1 (ab57834)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 28 2011