Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Very specific staining - even when used more concentrated the background was minimal. Staining very similiar to AB49405 and also to published IHC (PMID: 8896602).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Feb 20 2009