Application
Western blot
Sample
Mouse Tissue lysate - whole (Tibialis Anterior Muscle)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Tibialis Anterior Muscle
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 63 kDa
Additional data
Additional Notes
Both lane 1 and 2 are TA muscles from WT mice
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提交于 Feb 06 2018