抗金黄色葡萄球菌抗体(ab20920)
Key features and details
- Rabbit polyclonal to Staphylococcus aureus
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Staphylococcus aureus
- Isotype: IgG
概述
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产品名称
抗金黄色葡萄球菌抗体
参阅全部 Staphylococcus aureus 一抗 -
描述
兔多克隆抗体to金黄色葡萄球菌 -
宿主
Rabbit -
特异性
Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms. -
经测试应用
适用于: ICC/IF, IHC-Frmore details -
种属反应性
与反应: Staphylococcus aureus -
免疫原
Tissue, cells or virus corresponding to Staphylococcus aureus. Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (2) |
Use at an assay dependent concentration.
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IHC-Fr | (3) |
Use at an assay dependent concentration. Use with acetone fixed tissues.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. Use with acetone fixed tissues. |
靶标
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相关性
S.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
细胞定位
Secreted; cell wall. -
数据库链接
- Entrez Gene: 3919448 Staphylococcus aureus
- SwissProt: P02976 Staphylococcus aureus
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别名
- IgG binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
图片
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ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary
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ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10µg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
Red: cadherinBlue: DAPI
Green: anti-Staphylococcus aureus (ab20920)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (42)
ab20920 被引用在 42 文献中.
- Warheit-Niemi HI et al. Fibrotic lung disease inhibits immune responses to staphylococcal pneumonia via impaired neutrophil and macrophage function. JCI Insight 7:N/A (2022). PubMed: 34990413
- Li J et al. Fracture-related infection in osteoporotic bone causes more severe infection and further delays healing. Bone Joint Res 11:49-60 (2022). PubMed: 35100815
- Schultz D et al. Bioactive lipid screening during respiratory tract infections with bacterial and viral pathogens in mice. Metabolomics 18:39 (2022). PubMed: 35687250
- Leseigneur C et al. NAD kinase promotes Staphylococcus aureus pathogenesis by supporting production of virulence factors and protective enzymes. Elife 11:N/A (2022). PubMed: 35723663
- Filor V et al. Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices. J Dairy Sci 105:7705-7718 (2022). PubMed: 35879165