Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Tissue lysate - whole (Kidney)
Specification
Kidney
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Diluent: 3% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10
Detection
Detection method
ECL
Bands
Specific: ca. 60 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 05 2013