Application
Flow Cytometry
Sample
Human Cell (primary monocytes and macrophages)
Permeabilization
No
Gating Strategy
either monocytes or macrophages
Specification
primary monocytes and macrophages
Preparation
Cell harvesting/tissue preparation method: isolated from human peripheral blood
Sample buffer: DPBS + 2%FBS + 0.1% sodium azide
Sample buffer: DPBS + 2%FBS + 0.1% sodium azide
Fixation
live cells
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: DPBS + 2%FBS + 0.1% sodium azide
Concentration
5 µg/ml
Secondary antibody
Concentration
5 µg/ml
Name
Non-Abcam antibody was used: goat anti-rabbit IgG Alexa 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 14 2014