Anti-Sodium/Hydrogen Exchanger 1/NHE-1抗体(ab67314)
Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
- Suitable for: IHC-P, WB, ICC, ICC/IF, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-Sodium/Hydrogen Exchanger 1/NHE-1抗体
参阅全部 Sodium/Hydrogen Exchanger 1/NHE-1 一抗 -
描述
兔多克隆抗体to Sodium/Hydrogen Exchanger 1/NHE-1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC, ICC/IF, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1 (internal sequence). The immunogen is located within amino acids 490 - 540 of Sodium/Hydrogen Exchanger 1/NHE-1.
Database link: P19634 -
阳性对照
- WB: MOLT4 cell lysate. IHC-P: Human brain tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 2.5 µg/ml.
|
|
WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
|
ICC |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration. PubMed: 20817726
|
|
IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20712402
|
说明 |
---|
IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 20817726 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20712402 |
靶标
-
功能
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. -
组织特异性
Kidney and intestine. -
序列相似性
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 6548 Human
- Entrez Gene: 20544 Mouse
- Entrez Gene: 24782 Rat
- Omim: 107310 Human
- SwissProt: P19634 Human
- SwissProt: Q61165 Mouse
- SwissProt: P26431 Rat
- Unigene: 469116 Human
see all -
别名
- amiloride-sensitive antibody
- APNH antibody
- APNH1 antibody
see all
图片
-
Lane 1 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/ml
Lane 2 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 2 µg/ml
All lanes : MOLT4 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 48 kDa (possible isoform) -
Immunohistochemical staining of Sodium / Hydrogen Exchanger 1 in human brain tissue using ab67314 at 2.5 µg/ml.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (14)
ab67314 被引用在 14 文献中.
- Deguchi H et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy. Sci Rep 12:6263 (2022). PubMed: 35428816
- Lee HP et al. The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments. Sci Adv 7:N/A (2021). PubMed: 33523987
- Wang Y et al. Correlation between electrical characteristics and biomarkers in breast cancer cells. Sci Rep 11:14294 (2021). PubMed: 34253828
- Riaz S et al. Anti-hypertrophic effect of Na+/H+ exchanger-1 inhibition is mediated by reduced cathepsin B. Eur J Pharmacol 888:173420 (2020). PubMed: 32781168
- Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. Int J Mol Med 43:1193-1202 (2019). PubMed: 30592266