Anti-Smad2 (phospho S467)抗体(ab53100)
Key features and details
- Rabbit polyclonal to Smad2 (phospho S467)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Smad2 (phospho S467)抗体
参阅全部 Smad2 一抗 -
描述
兔多克隆抗体to Smad2 (phospho S467) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Smad2 (phospho S467).
Database link: Q15796 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Some lots contain 50% Glycerol, please contact our scientific support to confirm. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53100于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (9) |
1/300 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/300 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. -
组织特异性
Expressed at high levels in skeletal muscle, heart and placenta. -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. - Information by UniProt
-
数据库链接
- Entrez Gene: 4087 Human
- Omim: 601366 Human
- SwissProt: Q15796 Human
- Unigene: 12253 Human
- Unigene: 705764 Human
-
别名
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
- HsMAD2 antibody
see all
图片
-
All lanes : Anti-Smad2 (phospho S467) antibody (ab53100) at 1/300 dilution
Lane 1 : HeLa cell extract treated with PMA (125ng/ml, 15 mins)
Lane 2 : HeLa cell extract treated with PMA (125ng/ml, 15 mins) with phosphopeptide
Predicted band size: 58 kDa
Observed band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Smad2 (phospho S467) with ab53100. The image on the right is blocked with a phosphopeptide.
-
Anti-Smad2 (phospho S467) antibody (ab53100) at 1/750 dilution (for 16 hours at 4°C) + Mouse brain whole tissue lysate at 18 µg
Secondary
An HRP-conjugated Goat anti-rabbit-IgG polyclonal at 1/7000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at 18°C -
Anti-Smad2 (phospho S467) antibody (ab53100) at 1/1500 dilution + Rat lung whole tissue lysate at 15 µg with Milk at 5 %
Secondary
Goat antirabbit conjugated to HRP at 1/7000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
数据表及文件
-
SDS download
-
Datasheet download
文献 (93)
ab53100 被引用在 93 文献中.
- Wei W et al. Feibi decoction-medicated serum inhibits lipopolysaccharide-induced inflammation in RAW264.7 cells and BMDMs. Exp Ther Med 23:110 (2022). PubMed: 34976152
- Dugbartey GJ et al. Combination Therapy of Alpha-Lipoic Acid, Gliclazide and Ramipril Protects Against Development of Diabetic Cardiomyopathy via Inhibition of TGF-β/Smad Pathway. Front Pharmacol 13:850542 (2022). PubMed: 35401218
- Eryi S et al. HOXC6 Regulates the Epithelial-Mesenchymal Transition through the TGF-β/Smad Signaling Pathway and Predicts a Poor Prognosis in Glioblastoma. J Oncol 2022:8016102 (2022). PubMed: 35571491
- Balint B et al. SMAD3 contributes to ascending aortic dilatation independent of transforming growth factor-beta in bicuspid and unicuspid aortic valve disease. Sci Rep 12:15476 (2022). PubMed: 36104385
- Tian H et al. Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration. iScience 25:105050 (2022). PubMed: 36185374