Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (e16 T/S whole embryo body)
Specification
e16 T/S whole embryo body
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/250
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
In submitted image, no spinal cord positivity is seen. There is cytoplasmic staining of DRG cells and also a widespread, equally strong, immunostaining of developing skeletal muscles and osteoblasts, for example. In my opinion, this Ab does not give specific immunostaining in P.wax sections +/- HIER.
Abcam response
Many thanks for your IHC-P data on mouse tissue with ab10984; your data suggests that unfortunately this antibody looks unsuitable for use in mouse tissue IHC-P.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Mar 22 2010