重组Anti-Skp1抗体[EPR3303] (ab80586)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3303] to Skp1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Skp1抗体[EPR3303]
参阅全部 Skp1 一抗 -
描述
兔单克隆抗体[EPR3303] to Skp1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Skp1. The exact sequence is proprietary.
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阳性对照
- WB: lysates from 293, HeLa, PC12 or 3T3 cells; IHC-P: Human brain tissue; Flow Cyt (intra): permeabilized HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3303 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) | ||
WB | ||
IHC-P |
说明 |
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IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/1000 - 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the SKP1 family. - Information by UniProt
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数据库链接
- Entrez Gene: 6500 Human
- Entrez Gene: 21402 Mouse
- Entrez Gene: 287280 Rat
- Omim: 601434 Human
- SwissProt: P63208 Human
- SwissProt: Q9WTX5 Mouse
- SwissProt: Q6PEC4 Rat
- Unigene: 171626 Human
see all -
别名
- Cyclin A/CDK2 associated p19 antibody
- Cyclin A/CDK2 associated protein p19 antibody
- Cyclin-A/CDK2-associated protein p19 antibody
see all
图片
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All lanes : Anti-Skp1 antibody [EPR3303] (ab80586) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 ab80586.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by intracellular flow cytometry. Rabbit IgG (negative) is shown in green.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab80586 被引用在 2 文献中.
- Liu B et al. CHMP4C regulates lung squamous carcinogenesis and progression through cell cycle pathway. J Thorac Dis 13:4762-4774 (2021). PubMed: 34527317
- Wang D et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 109:9493-8 (2012). WB ; Mouse . PubMed: 22645357