Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Lumbar Spinal Cord section (30 micron thickness))
Specification
Lumbar Spinal Cord section (30 micron thickness)
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Normal Goat Serum 3% in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (Molecular Probes)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/500
Additional data
Additional Notes
Blocking was done by incubating the sections for 1h @ RT in NGS 10% + Triton 0.1% in PBS
Sections were also immunostained with Hoechst dye (blue) to highlight nuclei. The green signal is GFP expressed in motor neuronal cells.
Sections were also immunostained with Hoechst dye (blue) to highlight nuclei. The green signal is GFP expressed in motor neuronal cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Marco Peviani
Verified customer
提交于 Feb 23 2011