Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Purified protein (Soluble fraction)
Specification
Soluble fraction
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS tween 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL prime
Bands
Specific: 55 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
6 his tag protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 25 2014